#Research Microscope

[imosindia]

Microscope Manufacturers & Suppliers in India

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[karver518]

Something clicked in my mind, and so I decided to make a y/n for the Dreamweaver au for shits and giggles

Beginner weaver over here wish them luck

This cool bonus: their first day did not end well

Close up on the watcher's sphere Moonstone has (candy apple found them 💔💔💔🥲)

[ramshackledtrickster]

I cant take Sade seriously when he pops up in unity this is just grimy discount Preminger

[opens-up-4-nobody]

can I just say, I followed you for your beautiful art style and use of colours and I stayed for your beautiful art style and use of colours AND cyanobacteria. Like, I know nothing about them because I'm a classic lit major but more often than not I find myself reading your posts about cyanobacteria with unexpected interest and fascination

HAH! I'm glad u enjoy them bc I feel absolutely intolerable when I talk abt them lol. But also. I am the cyanobacteria evangelist. I am the patron saint of cyanobacteria.

[lionbearfox]

1 molecule

[cent-scratchnsniff]

it was just going to be a few warmup doodles but then she infected the rest of the page like the ever eternal and spreading spores. hod!!! hod. hod :)

[fancygremlin]

Sorry if I sound stupid but... does this mean that Clive was high while he was debriefing Warren in S1E6?!

Because that scene reminds me a lot of this one from S3E2 “2027”:

[duckydusky]

watching people make curly seem like a 1 dimensional character kills me like bro you DO NOT understand him like I do

[spottedgardeneelstan]

a criminally under discussed part of the extras

[lumi-wumi]

This is just a general post because I need to actually get my ideas in order to talk about this. But I was thinking about how some people say the sapphic ships are "male-gaze centric" in Black Sails because of how "it's focused" in sex or whatever. But then I was watching episode 4x01 (XXIX) with my friend yesterday. And realized... The thing that confirms to us that Silver and Madi have a relationship... Is a post sex scene. Jack and Anne in the beggining? "I wanna fuck". Miranda and Flint also in the beggining? Also confirmed by a sex scene. Vane and Eleanor I guess is the most hinted at before we actually get one of those but... We definitely got one of those as ultimate confirmation. Like... It's a constant theme. It's not something that's used with the sapphic characters only. Matter of factly, the only ship that does NOT have a sex scene in this show is James/Thomas which... I need to think about it a little further to try and understand why. And I don't mean it in a "this show is homophobic" way, but more like... If it's a constant and thematic, why? Black Sails is definitely NOT the show that would have tried to avoid a scene like this between two men - or even something post-sex like with Madi/Silver.

Idk, now I wanna rewatch it AGAIN and try to look at this from a thematic pov bc I don't think it's the kind of thing that you notice unless you stop and think of it. It only hit me bc I was bothered by how some people talk about the f/f ships in this show.

Just wanted to put that out there, I definitely wanna make a more detailed post about love and sex in this show when I put my thoughts in order with something more than WAIT A MINUTE, IT'S A THEME.

[pinkeoni]

streddit is such an interesting place because I’ve never seen a community that was so active in their fandom that so adamantly rejects what is happening on screen. Like it’s not a case of GA who watch the show casually so they may not pick up on certain things— it’s fans who frequently engage with the show, the supplemental books and comics, fan material, but they also so adamently ignore surface level canon material if it doesn’t match the version of the show they built in their head. They are too involved to be GA and let they know less than actual GA

[moonitoonz]

SPOILERS FOR CHAPTER 4

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i think dutch has petulant and impulsive bpd where his fps were hosea and arthur and after hosea was killed dutch internally latched more strongly to arthur which is why he immediately starts getting angry with him when arthur shows the slightest bit of disobedience because hes splitting and hahahahaha yeah but you guys arent ready for that conversation

[consult-sherlockholmes]

Hello, Mr. Holmes! How are you?

So, long story short, I ended up with an optical microscope in my room more or less 4 months ago, with 200 previously made slides (secured in a proper box), and lots of new ones too, for me to prepare myself. I love microbiology (it's one of my hyperfixations, curse my neurodivergency) and now I love it even more (my mother has had to drag me away from the microscope - I named it Wesley - in the middle of the night multiple times now).

After much conversation, I finally convinced my mom to buy me the proper equipment to prepare the slides!

So, I'm sending this ask to you, as I know you also have a microscope and that you use it a lot: what kind of equipment do you recommend me buying (gloves, scalpel blades, tints, etc), while still remembering that all of the stuff needs to stay in my room (properly taken cared of by me, of course)?

For example, I'm unsure if different dyes are used for different smears and specimens due to it's affinity (I've noticed that on 'organic matter' slides, images are usually tinted purple or pink, while on plant-based slides, images are usually tinted green and blue, with a few red structures.) Considering that I don't have access to a mortuary, I will mostly make plant slides. There must be a difference in the dyes then, right?

Sorry for the long text! Hope this isn't too much of a bother.

- a 17-year-old :)

Congratulations on your new light microscope. I do hope you get the best out of it. I am overjoyed that someone else appreciates the art of microscopy and microbiology.

However, you need to be careful to not strain your eyes. It is recommended to take breaks every 15 minutes to close your eyes or focus on something in the distance to reaccommodate your eyes. And get up every 40 minutes, stretch and correct your posture. And it is recommended to not use a microscope more than 5 hours per day. John has to chase me away from my microscope sometimes to take a break when I sit there for hours, my posture like a Caridea.

Concerning equipment, you will obviously need a scalpel or other sharp blade to make very thin slices of your specimen, as thin as possible. And forceps to move your samples (best just get a whole dissection kit it has everything). Obviously slides and coverslips, pipettes for the stains or water, maybe some tubes. A pen to label your slides. In many staining procedures ethanol or acetone is also used. A waste jar to safely dispose of any chemicals, but be careful what you mix. A rack for staining and containers. I would recommend nitrile gloves, some people are sensitive to latex.

The dyes you use depend on the specimen. For example in histological slides of tissues hematoxylin and eosin are most commonly used (short HE-stain). That's what you most likely saw on your slides, it's blue, purple and pink. Hematoxylin is a basic compound extracted and oxidised from the logwood tree (Haematoxylum campechianum), and it stains acidic compounds in the cells (or basophilic because they have an affinity for basic substances). For example nucleic acids like DNA or RNA get stained by hematoxylin because they are basophillic. And where are lots of nucleic acids? In the nucleus and ribosomes, that is why they appear blue to purple in the staining because they bind hematoxylin. Eosin is an acidic compound, and stains basic or acidophilic compounds red or pinkish, like proteins, collagen, cytoplasm, extracellular matrix.

(Ductus epididymidis with HE-stain)

(Tongue HE-stain, pointer marking a ganglion; that is my picture)

Of course there are more specific stains for specific tissues like Golgi's silver staining for neurons.

For plants toluidine blue is often used, high affinity for acidic tissues, and can stain blue to green to purple. It is often combined with safranin, a basic azine, which is probably the red stain you saw. It stains polysaccharides and lignin, woody parts of the plant. Safranin and astrablue is also often combined, astrablue stains non-lignified parts of the plant.

(Ulex europaeus stem; not my pictures I don't have any samples currently, source Atlas of plant and animal histology)

Safranin is also used in bacteriology, in the famous Gram staining. In Gram staining you use crystal violet (blue/purple), Lugol's iodine solution, then wash it with ethanol and add safranin (red) as a counter stain. Bacteria is gram-positive if the crystal violet stays in their thick murein cell wall, can't be washed out with the ethanol and the bacteria stays blue. Gram-negative appear red because of the counterstain.

(Staphyloccocus aureus (violet, gram positive) & Escherichia coli (red, gram negative); not my picture, source Wikipedia)

However, I am not sure whether you have access to any of those substances, if they are too expensive for you or if they are too hazardous if used in your own room for a prolongued time. Of course those substances need to be stored properly, and your own room is probably not a good place, especially for ethanol or acetone. The fumes. I would recommend to ask your biology or chemistry teacher whether they can recommend anything further and where to buy said solutions in your area, and if they can't they are idiots. There are also many useful resources and tutorials on Youtube.

Another fascinating experiment for your microscope, that you can perform without buying any chemicals, is a hay infusion. You put hay into a container filled with water, and let it sit undisturbed for a week in a sunny area but not in direct harsh sunlight. During that time the microorganisms in the hay are reproducing in the solution, feeding on the polysaccharides of the hay. Protozoans also flourish in the hay infusion and eat the bacteria. It might get cloudy and a bit foul smelling (best not do it in your own room if you don't want to sleep next to a rotting smell). When you put a drop of the solution onto a slide and look at it in the microscope, you should see a variety of microorganisms like bacteria (like Bacillus subtilis), amoeba, ciliates, heliozoa, algae et cetera. At different depths of the liquid you should find different kinds of organisms, because of differing oxygen content. However, pathogens can also occur in the hay infusion so handle it carefully and work sterile, wash your hands properly.

And even if you don't work at a morgue you can still get tissue samples to experiment on, after all meat is sold in supermarkets, basically the same as a human body. And at the butchers they even sell organs like chicken hearts, pig kidney, liver, blood et cetera. Or observe your own hair under the microscope.

Which kind of samples and slides were included in your starter kit? Be careful to not leave them lying around in the sunlight, or the stain might fade. Always store them in the proper box.

[mushrooms-and-millipedes]

Good days work of making an ant graveyard

[borealopelta]

wip whatever

i'm gonna go ahead and consider myself tagged in @arokel's tagless tag game because i want to talk about My Wips :-) if anyone feels like joining in, please do!!

after posting my latest fic i was kinda like. hmm. i don't have anything to show. which is not true!! i have not one but two geologist!don fics in the wip folder. (now you could ask. hey finch why do you keep writing geologyfic. well a) i have this mental illness called degree in geology and b) it's basically just me trying to conceptualise what being a geologist (or studying geology) must have been like for someone who was doing it before the theory of plate tectonics was even proposed (not to mention accepted). the amount of stuff that was fully unknown to geology in the times these fics are set is limiting as fuck and that's what makes them endlessly fun to tinker with. i've done sooooo much niche research. this is what constitutes fun for me. apologies)

i've already talked about the first one in an ask game, don's horrible geology exam, which i've been slowly piecing together from semi coherent notes app scribblings from the height of my mystery illness, BUT the other is newww so. also i don't have titles picked out because i'm bad at that so the titles are. the file names. yay! snippets under readmore:

geologydonfic is the aforementioned don's exam misery fic

Bobby takes in Don – the line of his bracelet a red imprint on his cheek, bruise-black circles under his red-rimmed eyes, paler than ever, frown lines like deep etchings on his handsome face. It’s clear that he’s not dealing on his own. “Fine,” he says after barely any deliberation, “I can help you study.” He picks up Don’s textbook and flips to a random page. Most of it is taken up by a big, colourful chart, sort of like a repeating rainbow getting more and more washed out, with ray-like lines coming out of one corner. It’s utterly incomprehensible. Bobby puts the book back down on the table. “I can’t help you study.” The corner of Don’s mouth twists into an almost-smile.

argentina is the newer one, set in argentina in 1967. don is there for work, bobby is there for don, it's hot, it's dirty (so far only in the "the drill site is a filthy place in general" way but who knows)

The Jeep that had picked him up at the airport slowly rolled away to join the fleet of identical vehicles, all decorated with the letters YPF – the name of the company in charge of the work, Bob assumed. He barely had time to feel (or look) lost when he noticed Don coming his way. Or at least he guessed it was Don, from the familiar walk and the fact that the man was a good four inches taller than everyone around him. Dressed in grubby work clothes and wearing a hard-hat that cast enough shadow to hide his entire face however, he could have been anyone. Then the smile gave him away. Bob had not been twenty-two and a fool in love in a good while, but that toothpaste-commercial-runaway, all-teeth, bright-as-the-sun smile Don could smile when he really put his mind to it still made him go weak in the knees.

[science-lings]

love it when i find something to be weird and bullshit science about, truly my favorite part of blatantly non-realistic fandoms. i honestly love comic book/ fantasy science bc you can fake it into making sense even if that is not how things work. Anyway I want to write a fic where Bruce tries to build up an immunity to scarecrow's fear toxin and I get to extensively write about its effects bc I think Bruce should be nerdier